PhD Student in Chromatin-Associated lncRNAs Regulating Seed Dormancy

This PhD position at the Institute of Biochemistry and Biophysics, Polish Academy of Sciences, focuses on uncovering how chromatin-associated long non-coding RNAs (lncRNAs) regulate seed dormancy and transcription in plants. The project builds on recent discoveries linking *MUSHER* lncRNA to the *DOG1* gene and ABA signalling pathways, aiming to clarify its role in chromatin activation and nuclear retention. Using CRISPR mutagenesis, transcriptomics, ChIP-seq, and spatial genomics, the candidate will investigate the functional consequences of lncRNA-chromatin interactions under diverse environmental conditions. The research seeks to advance mechanistic insights into lncRNA-mediated gene regulation and adaptive seed behaviour, with potential implications for plant biology and agriculture.

• Investigate the role of chromatin-associated lncRNAs in regulating seed dormancy and transcription in plants.
• Apply CRISPR mutagenesis to study functional consequences of lncRNA-chromatin interactions.
• Conduct transcriptomics and ChIP-seq analyses to elucidate lncRNA-mediated gene regulation mechanisms.
• Clarify nuclear retention mechanisms of lncRNAs and their impact on chromatin binding.
• Analyze physiological, genetic, and molecular responses of seeds to environmental cues.
• Publish findings in high-impact scientific journals and present results at international conferences.
• Collaborate with the research group to integrate findings into broader plant biology research.