PhD Position in Biophysical Origins of RNA Toxicity in Huntington’s Disease

This PhD position at Utrecht University focuses on uncovering the biophysical origins of RNA toxicity in Huntington’s Disease (HD). While HD research has traditionally centered on the toxic polyglutamine protein, recent discoveries highlight the role of HTT mRNA condensates and aggregates in neuronal toxicity. The successful candidate will design and execute single-molecule experiments using optical tweezers and confocal fluorescence imaging, building on prior work (O’Brien et al., *Nature Communications*, 2024). The project bridges biophysics and biochemistry to elucidate why certain repeat RNAs are toxic while others are not, with potential therapeutic implications. The candidate will work under the mentorship of Prof. Christian Kaiser in an interdisciplinary environment at the Bijvoet Centre for Biomolecular Research.

• Design and execute single-molecule experiments using optical tweezers and confocal fluorescence imaging to study RNA toxicity mechanisms.
• Perform independent experimental work, analyze data, and interpret results to advance understanding of molecular toxicity in HD.
• Present research progress regularly in team meetings and at scientific conferences.
• Publish findings in peer-reviewed journals to contribute to the broader scientific community.
• Support teaching and mentoring undergraduate and master’s students to foster interdisciplinary collaboration.
• Collaborate with junior investigators and international partners to develop novel experimental approaches for RNA and protein labeling.